Gallery

Separase in Exocytosis

Separase localization dynamics during meiosis. sep-1::gfp, h2b::mCherry, cpg-2::mCherry (cortical granules)

Visualization of cortical granules. Embryo was expressing histone::GFP and labeled with plasma membrane dye FM2-10.

Details forthcoming

Expressing cpg-1::mCherry; H2B::mCherry; sep-1::GFP, under 36 hr of ify-1 RNAi at 20°C

In utero movie of meiosis I in C. elegans. The fluorescent proteins visualized are endogenously tagged, sep-1::mCherry; cav-1::GFP; his-72::mTurquoise2.

Midbody, Development, Cytokinesis

Lattice Light Sheet Imaging of endogenous Aurora B Kinase and non-muscle myosin II in C. elegans

air-2::GFP; H2B::mCherry; PH::mCherry

Expressing air-2::GFP, H2B::mCherry. Midbody formation and labeling of intestinal lumen, pharynx, sensilla dendrites.

air-2::mScarlet, mitosis and midbody formation